마이크로그루브를 부여한 티타늄합금디스크에 대한 인간치은상피세포와 인간치은섬유아세포의 세포-기질 작용에 관한 주사전자현미경 연구
Cell-matrix interactions of human gingival epithelial cells and fibroblasts with microgrooved titanum alloy substrata : a scanning electron microscopic study
이석원, 류인철, 김경화, 한종현, 허성주,
소속 상세정보
이석원 ( Lee Suk-Won ) - 연세대학교 치과대학 보철과학교실
류인철 ( Rhyu In-Chul ) - 서울대학교 치과대학 치주과학교실
김경화 ( Kim Kyoung-Hwa ) - 서울대학교 치과대학 치주과학교실
한종현 ( Han Chong-Hyun ) - 연세대학교 치과대학 보철과학교실
허성주 ( Heo Seong-Joo ) - 서울대학교 치과대학 보철과학교실
KMID : 0602720040080020002
Abstract
Forming horizontal microgrooves on the surface of transmucosal abutment connected to implant is one of the several methods to enhance initial cell attachment to the abutment immediately after abutment connection procedure.
The purpose of the present study is to observe attachment, proliferation and migration of human gingival epithelial cells and fibroblasts on microgrooved titanium alloy substrata. Healthy human gingival epithelial cells(HGEs) and human gingival fibroblasts(HGFs) were obtained. To produce cell-matrix substrata, titanium alloy discs with various widths of microgrooves were fabricated using photolithography technique. Flat titanium alloy discs were used as control. The cultured cells were seeded and incubated for 3 days followed by scanning electron microscopic(SEM) observations. On SEM observations, HGEs, as a thin epithelial sheet, proliferated and migrated along the microgrooves, but with little tendency. HGFs proliferated and migrated along the microgrooves, and on which the widths of the microgrooves were over 15μm, had the tendencies to form thick rod-like, elongated structures. The differences in the form and structure of the observed cells on different widths of the microgrooves indicate that the microgrooves formed on titanium alloy substrata change the behaviors of human gingival epithelial cells and fibroblasts in vitro. According to the results of the present study, it is suggested that the microgrooves on dental implant abutments ,by increasing fibroblasts' and epithelial cells' attachments, proliferations, and migrations, may play a role in enhancing the quantity and the quality of the soft tissue around dental implants. A further study using more detailed models on molecular levels to verify the results of the present pilot study is strongly required.
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